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. 2019 Nov 5;10:1380. doi: 10.3389/fphys.2019.01380

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Copyright © 2019 Montanari, Boschi and Colitti.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative images (ROI) of immunoreactivity in CTRL and 1CP-treated 3T3-L1 cells after 8 days of differentiation (8d). (a) PLIN1 positivity along the large LDs in CTRL cells; (b) 1CP-treated cells showed PLIN1 immunoreactivity observed along the small and large LDs (c) no UCP1 immunoreaction was observed in CTRL cells (d) granular UCP1 cytoplasmic positivity in 1CP- treated cells; (e) no TRPV1 immunoreaction was observed in CTRL cells (f) 1CP-treated cells showed membrane and cytoplasmic TRPV1 immunostaining. Nuclear staining DAPI. Images are representative of n. 5 biological replicates. Scale bars: 5 μm.