TABLE 3.

Differentially expressed genes in X9 cells at 4 and 8 days.

Treatment 4d	Adrb3		SEM	Ucp1		SEM	Tbx1		SEM	Trpv1		SEM	Prdm16		SEM	Ppargc1a		SEM	Cidec		SEM	Cidea		SEM	Plin1		SEM
NE	8.70A	±	1.24	3.34A	±	0.45	2.10	±	0.33	1.57	±	0.39	3.04	±	0.64	2.16AB	±	0.50	1.90	±	0.28	1.34	±	0.22	1.64	±	0.35
1CP	0.66B	±	1.25	0.94AB	±	0.45	0.92	±	0.33	1.16	±	0.40	0.95	±	0.65	4.07A	±	0.51	1.38	±	0.28	1.12	±	0.23	0.22	±	0.36
0.1CPNE	3.04AB	±	1.53	2.12AB	±	0.55	0.81	±	0.41	1.28	±	0.49	1.66	±	0.79	1.86AB	±	0.62	0.56	±	0.35	0.87	±	0.28	0.08	±	0.44
CTRL	0.96B	±	1.25	0.95AB	±	0.45	1.03	±	0.33	1.15	±	0.40	1.04	±	0.65	1.18B	±	0.51	1.00	±	0.28	1.13	±	0.23	1.15	±	0.36
0.1CP	0.64B	±	1.25	0.52B	±	0.45	0.71	±	0.33	0.73	±	0.40	0.84	±	0.65	2.79AB	±	0.51	1.25	±	0.28	1.37	±	0.23	0.42	±	0.36
1CPNE	2.44AB	±	1.25	0.93AB	±	0.45	0.69	±	0.33	1.12	±	0.40	2.69	±	0.65	1.68AB	±	0.51	0.44	±	0.28	0.74	±	0.23	0.09	±	0.36
Pr > F (Model)	0.007			0.018			0.088			0.790			0.136			0.029			0.039			0.383			0.064
Treatment 8d	Adrb3		SEM	Ucp1		SEM	Tbx1		SEM	Trpv1		SEM	Prdm16		SEM	Ppargc1a		SEM	Cidec		SEM	Cidea		SEM	Plin1		SEM
CTRL	1.00A	±	0.07	0.66B	±	0.52	1.05	±	0.12	1.06	±	0.26	0.78B	±	0.33	1.02	±	0.14	1.02ABC	±	0.17	1.03AB	±	0.18	0.92A	±	0.13
NE	0.16B	±	0.07	3.59A	±	0.50	0.92	±	0.12	1.62	±	0.26	2.83A	±	0.33	0.95	±	0.14	0.60BC	±	0.17	0.50B	±	0.18	0.35AB	±	0.12
1CP	0.78A	±	0.07	1.11AB	±	0.52	0.72	±	0.12	0.99	±	0.26	0.82B	±	0.33	0.76	±	0.14	1.32AB	±	0.17	0.57B	±	0.18	0.66AB	±	0.13
0.1CP	0.96A	±	0.07	0.66B	±	0.52	0.58	±	0.12	0.74	±	0.26	0.56B	±	0.33	0.90	±	0.14	1.87A	±	0.17	1.89A	±	0.18	0.85A	±	0.13
0.1CPNE	0.14B	±	0.07	1.60AB	±	0.52	0.60	±	0.12	0.74	±	0.26	0.84B	±	0.33	0.44	±	0.14	1.04ABC	±	0.17	0.27B	±	0.18	0.37AB	±	0.13
1CPNE	0.09B	±	0.07	2.15AB	±	0.52	0.58	±	0.12	1.52	±	0.26	2.09AB	±	0.33	0.58	±	0.14	0.24C	±	0.17	0.12B	±	0.18	0.10B	±	0.13
Pr > F (Model)	<0.0001			0.015			0.072			0.125			0.002			0.088			0.000			0.000			0.005

CTRL = vehicle negative control; NE = 10 μM norepinephrine; 0.1CP = 0.1 μM capsaicin; 0.1CPNE = 0.1 μM capsaicin plus 10 μM norepinephrine; 1CP = 1 μM capsaicin; 1CPNE = 1 μM capsaicin plus 10 μM norepinephrine. Adrb3, adrenoceptor beta 3; Ucp1, uncoupling protein 1; Tbx1, T-box 1;Trpv1, transient receptor potential vanilloid 1; Prdm16, proline rich domain containing 16; Ppargc1a, peroxisome proliferator-activated receptor γ coactivator 1 α; Cidec, cell death inducing DFFA like effector C; Cidea, cell death-inducing DFFA-like effector A; Plin1, perilipin 1. 4d = at 4 days of differentiation; 8d = at 8 days of differentiation. The data are shown as Least squares (LS) means ± standard error of the mean (SEM). Pr > F (Model) = p-value for the effect of the model on the response. Capital letters indicate a significant Bonferroni-corrected difference (p < 0.003).