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. 2019 Nov 11;9:16460. doi: 10.1038/s41598-019-52965-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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High frequency measurement of OHC NLC in organ of Corti explants. (A) Light micrograph of an in situ patch clamped OHC. sc, stereocilia; n, nucleus; pip, patch pipette. (B) Whole cell currents induced by chirp voltage protocol. (C) Derived voltage-dependent capacitance (circles) at each frequency (see methods) was fit to extract NLC and its Boltzmann parameters. (D,E) Surface plots of raw C_m data before (D) and after (E) correction for residual stray capacitance effects on high frequency measures. Note frequency independence of C_lin following correction.