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. 2019 Nov 11;9:16460. doi: 10.1038/s41598-019-52965-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Boltzmann characteristics (mean +/− se) for selected bandwidths. The dashed line denotes NLC measured at low frequencies from isolated OHCs (Abe et al., 2010). Note abrupt decline of C_lin, while C_sa remains steady. V_h is initially at a more positive potential than isolated cells. Q_max (pC) and Q_sp (pC/pF) increase rapidly during development. Q_max decreases after p17/18, with a clear difference between low and high frequencies, but Q_sp stabilizes because of the C_lin drop. Following some variability at p6/7, z increases with age, and also tends to be smaller at higher frequencies. P6,7 n = 7; p8,9 n = 7; p12,13 n = 6; p17,18 n = 8; 2 month n = 8; 8 month n = 9.