Table 2.
PBED matrix comparison between T4 and T11 for the observed and predicted results
| Treatment | Factor type | Volume of culture media (mL) | CuSO4 (mM) | Inoculum (% v/v) | Glucose (g L−1) | (NH4)2SO4 (mM) | Peptone (g L−1) | Yeast extract (g L−1) | Observed Enz Act. (U L−1) 168 h | Predicted Enz Act. (U L−1) 168 h |
|---|---|---|---|---|---|---|---|---|---|---|
| T4 | Factorial | 150 | 1 | 10 | 10 | 20 | 20 | 10 | 1254.270 | 1276.130 |
| T11 | Factorial | 300 | 1 | 10 | 10 | 5 | 10 | 10 | 1373.720 | 1276.130 |
| Center point | 225 | 0.55 | 6 | 20 | 12.5 | 15 | 7.5 | 1151.880 | 975.363 | |
| Center point | 225 | 0.55 | 6 | 20 | 12.5 | 15 | 7.5 | 1275.600 | 975.363 | |
| Center point | 225 | 0.55 | 6 | 20 | 12.5 | 15 | 7.5 | 1339.590 | 975.363 | |
Defined center points as consequence of factors influencing laccase activities. Factors at two levels in addition to a center point, as described in “Materials and methods”