Fig. 1.

Development and molecular characterization of CsAlaAT2 transgenic rice event. a Schematic diagram of the T-DNA construct OsAnt1::CsAlaAT2, containing the CsAlaAT2 gene with the NOS terminator under the control of the OsAnt1 promoter. RB, right border; prOsAnt1, promoter antiquitin1; CsAlaAT2, Cucumis sativus Alanine aminotransferase; tNOS, terminator Nopaline synthase; pr 35 S, promoter 35 S; hpt, hygromycin phosphotransferase; C35S, cauliflower 35 S; LB, left border. b PCR amplification of cucumber AlaAT on T0 transgenic rice lines. M = 1 kb DNA ladder plus; 1–14 = transgenic lines, WT = wild type, P = plasmid control. c Southern blot analysis showing the copy number of hpt gene insertion in the T0 generation of transgenic lines. Hpt gene insertion is shown by white arrow. M = marker 1 kb ladder, WT = wild type, 1 = FAM3, 2 = FAM4, 3 = FAM5, 4 = FAM 9, 5 = FAM13, P = plasmid of pCAMBIA1301-CsAlaAT2