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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2019 Jul 25;143:16–24. doi: 10.1016/j.freeradbiomed.2019.07.020

Figure 2: Effect of Varying Cell Protein Sample and Measurement Time on Apparent Immuno-Proteasomal Proteolysis.

Figure 2:

Different amounts of cell protein were loaded in order to test the proteolytic response of the Immuno-Proteasome over time in each cell type (A, B, C, SAECs; D, E, F, Wi38; G, H, J, MEFs). Different amounts of cell protein resulted in different apparent Immuno-Proteasomal activities. Kinetic curves provided a range in which to find a linear response (i.e. when the rate of reaction was constant). The results shown here are the mean ± SD of at least 3 independent trials.