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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2019 Jul 25;143:16–24. doi: 10.1016/j.freeradbiomed.2019.07.020

Figure 3: The Impact of Assay Duration and Sampling Frequency on Apparent Trypsin-like Activity of the Proteasome in SAEC cells.

Figure 3:

A: An extended assay with infrequent measurement intervals results in an imprecise linear range that leads to a miscalculation of the specific activity. B & C: A shorter duration of the assay with increased frequency of measurement intervals result in more precise determination of specific activity. D: Specific activity calculated from assays of different duration and, with different frequencies of measurement. The results are the mean ± SD of at least 3 independent trials.