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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2019 Jul 25;143:16–24. doi: 10.1016/j.freeradbiomed.2019.07.020

Figure 4: Creating an AMC Standard Curve Against which to Calibrate Rates of Proteolysis.

Figure 4:

A: The AMC standard curve has a logarithmic distribution with saturation at high concentrations. B: Low AMC concentrations provide a linear curve well suited for correlating fluorescence to units of AMC, which can then be used to determine proteolytic activity from free AMC. The results showed here are the mean ± SD of at least 5 independent replicates.