Table 5:
MicroRNAs used to treat OA in rodents using intra-articular delivery
| MicroRNA | Expression | Species | Model | Analysis time point | Outcome | Target gene | Reference |
|---|---|---|---|---|---|---|---|
| miR-140 | Agomir | Rat | ACLT+MMx | 4 weeks, 8 weeks, 12 weeks | Increased cartilage anabolism; reduced cartilage pathology; decreased Mmp13 and Adamts4 expression | Mmp13, Adamts4 | [143] |
| miR-181a-5p | Antisense oligonucleotide | Rat | DMM | 10 weeks | Attenuated cartilage destruction; decreased expression of catabolic, hypertrophic and apoptotic genes; reduced collagen type II breakdown | Mmp13, Col10, Parp, p85, Casp3 | [144] |
| miR-93 | Agomir | Mouse | MMT | 2 weeks | Inhibited levels of Il1b, Tnfa, and Il6; deceased chondrocyte apoptosis | Tlr4, Nfkb | [145] |
| miR-29a | Lentivirus | Mouse | CIA | 8 weeks | Lessened the collagenase aggravation of excessive synovial remodeling reaction; lowered Vegf production and angiogenic activation | Vegf | [146] |
| miR-483-5p | Antagomir | Mouse | DMM | 5 weeks | Decreased cartilage pathology score; marked reduction in Runx2 positive chondrocytes | Matn3 | [147] |
| miR-98 | Antagomir | Rat | ACLT+MMT | 2 weeks | Relieved cartilage degradation; prevented downregulation of Bcl2 in cartilage | Bcl2 | [148] |
| miR-222 | Lentivirus | Mouse | DMM | 8 weeks | Reduced cartilage destruction; decreased Mmp13 levels | Mmp13 | [149] |
miR = microRNA; ACLT = anterior cruciate ligament tear; MMx, total medial meniscectomy; DMM = destabilization of medial meniscus; MMT = medial meniscus tear; CIA = collagen induced arthritis