Representative western blot bands of time course (A) and densitometric quantification of TGR5 (B) in the ipsilateral hemisphere after SAH. Data was represented as mean ± SD. n=6 per group. *P<0.05 vs. Sham group; One-way ANOVA, Tukey’s post hoc test. (C) Representative microphotographs of co-immunofluorescence staining of TGR5 (red) with neurons (NeuN, green), astrocytes (GFAP, green), and microglia (Iba-1, green) in the ipsilateral basal cortex at 24 h after SAH. Nuclei were stained with DAPI (blue). A small black square within coronal section of brain indicated the location of where the immunofluorescence staining images were taken. n=2 per group.