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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2019 Sep 4;143:441–453. doi: 10.1016/j.freeradbiomed.2019.09.002

Figure 7. Effects of CRISPR knockout of TGR5 and ALDH2 on neurobehavioral outcome at 24 h after SAH.

Figure 7.

(A-B) TGR5 CRISPR and ALDH2 CRISPR pretreatment reversed the neurobehavioral benefits of INT-777 at 24 h after SAH, n=6 per group. (C-E) Representative western blot bands and densitometric quantification of TGR5 and ALDH2 demonstrated the efficacy of CRISPR knockout in naive and SAH rats, n=4 per group. Vehicle: 10% dimethyl sulfide. Data was represented as mean ± SD. *P<0.05 vs. Sham group; @P<0.05 vs. SAH+Vehicle group; &P<0.05 vs. SAH+scr CRISPR group; #P<0.05 vs. SAH+scr CRISPR group; One-way ANOVA, Tukey’s post hoc test; scr CRISPR, scrambled CRISPR; KO CRISPR, Knockout CRISPR.