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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2019 Jul 26;143:25–46. doi: 10.1016/j.freeradbiomed.2019.07.024

Figure 5. Control of ATF3 transcript variant 4 and 5 on lipolysis-induced NRF2 signaling-related gene expression.

Figure 5.

HBMECs were transfected with ATF3-T4 or ATF3-T5 siRNA for 18 h and treated with TGRL lipolysis products (TL) for 3 h. mRNA expression was determined by qRT-PCR. The expression of each gene was normalized to that of GAPDH and the fold change was calculated as the difference in expression with TGRL lipolysis products in the presence of scrambled siRNA and ATF3-T4 siRNA or ATF3-T5 siRNA. A) NRF2 and PTGS2/COX-2 were significantly suppressed and HOX-1 was significantly increased after transfection with ATF3-T4 siRNA and followed by treatment with TL (N = 3/treatment group, *P≤ 0.05 compared to scrambled TL); B) PTGS2/COX-2 were significantly suppressed and HOX-1 was significantly increased after transfection with ATF3-T5 siRNA and followed by treatment with TL (N = 3/treatment group, *P≤ 0.05 compared to scrambled TL).