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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Free Radic Biol Med. 2019 Jul 26;143:25–46. doi: 10.1016/j.freeradbiomed.2019.07.024

Table 7.

Summary of TGRL lipolysis products and ATF3 transcript variant 4 or 5 siRNA treatment in HBMECs

Treatment Response
TGRL Lipolysis Products mRNA expression
Up-regulation of CHAC1, DDIT4, ATF3, DDIT3, JDP2, CCL20, CEBPB, CXCL3.
Up-regulation of TNF signaling (CSF2, FOSL1, FOSL2, LIF, ATF4, SELE, NFKB1, NFKBIA, JUNB, JUN)
Up-regulation of NRF2 signaling (EIF2A3K, NRF2, HIF1α, HOX-1, SOD-2, PTGS2/COX-2)
Up-regulation of Inflammation (IL-8, IL1α, VEGFA, ICAM-1, HSPA6, GADD45A, IL-6, CXCL-2, ADAM-2, KLF4)
Up-regulation of Transcript variant 4 and 5 of ATF3.
Protein Expression
Up-regulation of ATF3, ATF4, p-c-Jun, JDP2, JunB, CHAC1; TNFR1, DR5; NRF2, COX-2.
Apotosis
Increase Cleaved caspase-9, Active caspase-3, Cleaved PARP
ATF3-T4 siRNA + TGRL Lipolysis Products mRNA expression
Up-regulation of CHAC1, JDP2, CEBPB; FOSL1; HOX-1.
Down-Regulation of CCL20; CSF2, SELE; NRF2, COX-2; IL-8, IL-1α, ICAM-1, IL-6, CXCL-2.
Protein Expression
Up-regulation of ATF4, JDP2, CHAC1, TNFR1.
Down-Regulation of JunB, DR5, NRF2, EIF2α, PTGS2/COX-2.
Apotosis
Decrease Cleaved caspase-9, Active caspase-3; No change of Cleaved PARP; Increased p53.
ATF3-T5 siRNA + TGRL Lipolysis Products mRNA expression
Up-regulation of FOSL1, FOSL2, JunB, Jun; HOX-1; IL-1α.
Down-Regulation of CHAC1; COX-2; IL-6.
Protein Expression
Up-regulation of ATF4, p-c-Jun.
Down-Regulation of JunB, CHAC1, DR5, TNFR1, NRF2, EIF2α, COX-2.
Apotosis
Increased Cleaved caspase-9; Decreased Active caspase-3, Cleaved PARP, p53.
CPT1A1 siRNA + TGRL Lipolysis Products mRNA expression
Down-Regulation of NRF2 signaling (EIF2A3K, NRF2, HIF1a, HOX-1, PTGS2/COX-2)
Down-regulation of Transcript variant 4 and 5 of ATF3.
Down-Regulation of Inflammation (IL-8, SELE/E-Selectin)
No Change SOD-2 and IL-6