Fig. 3.

Ser456-to-Leu mutant proteins are minimally localized in the ER. (A) COS-7 cells were transfected with the plasmid encoding the mutant (green) and were immunostained with an anti-KDEL antibody (red). The merged image (yellow) is also shown. Dotted outlines indicate the periphery of the cell. (B) Fluorescence intensities (F.I., arbitrary unit) of green and red along the dotted lines (left upper-to-right down direction) in the upper left and right images of panel A are shown, respectively. (C) Percentages of merged yellow pixels per mutant green ones are statistically shown (n = 3 fields).