Fig. 7.

Expression of Ser456-to-Leu mutant constructs decreases phosphorylation of ribosomal S6 proteins in an oligodendroglial cell line. (A) FBD-102b cells were transfected with the plasmid encoding the mutant (green) and were immunostained with an anti-phospho-S6 antibody (red). The merged image is shown. Dotted outlines indicate the periphery of the transfected cells, which have exhibited decreased S6 phosphorylation (B) Percentages of phosphorylated S6 in cells expressing the mutant proteins or not are statistically shown (**, p < 0.01 of one-way ANOVA followed by a Fisher's protected least significant difference test as a post hoc comparison; n = 3 fields).