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. 2019 Oct 24;105(5):1048–1056. doi: 10.1016/j.ajhg.2019.09.025

Figure 4.

Figure 4

Ntng2 Knockdown in N2a Cells

(A) Knockdown of endogenous Ntng2 by Ntng2-specific siRNAs as normalized to control siRNA. Results of quantitative RT-PCR 30 h post-transfection.

(B–D) Effects of Ntng2 knockdown on neurite outgrowth. Data presented as mean and SD; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Quantification was conducted by counting the absolute number of cells with neurites (B), measuring the neurite length by NeuroLucida tracing (C), and quantification of the Convex hull area (D). All analyses show a significant reduction of neurite number and length as a consequence of Ntng2 knockdown.

(E–F) Example of the N2a appearance at 30 h post-transfection with control (E) and Ntng2-specific siRNA (F). Visualization was done using MAP2 counterstaining (red).