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. 2019 Nov 12;19:21. doi: 10.1186/s12861-019-0202-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Microscopic analysis and apoptosis assay in a representative Stage 22 unisexual Ambystoma embryo. a and d Hoechst stain (blue fluorescence) indicates locations of cell nuclei. b Unisexual positive control, treated with TACS-NucleaseTM (Trevigen), a proprietary endonuclease, exhibits positive TUNEL staining as is evident by green fluorescence indicating the presence of DNA damage. e Experimental unisexual sample exhibits negative TUNEL staining (no green fluorescence), indicating absence of DNA damage characteristic of apoptosis. c and f Hoechst stain and TUNEL labeling merged with phase contrast microscopy