Fig. 5.

Phosphorylation of S25 and S1201 are involved in the peripheral nerve injury in adult mouse. a, b and c Immunofluorescence of mouse longitudinal sciatic nerve sections, using pS25 (a), pS1201 (b), or SCG10 (c) Abs (each green) and Tuj-1 (red) Ab, at 6 h or in 2 days after injury. The proximal side (left) and the injured point (arrowhead) are shown. Intact: sham operation in the other side of the injured nerve. Scale bar: 500 μm. d Regeneration index [23], which is the distance from crush site to the location that the signal level of target object decreases to the half of the crush site, of pS25 and pS1201 was higher on 2 days after crush than on 6 h after crush. The index of pS25 and pS1201 was similar to the index of SCG10. N = 3 (6 h) and n = 3 (2 d); ***p < 0.001 by one-way ANOVA with Sidak’s multiple comparisons test