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. 2019 Nov 11;12:93. doi: 10.1186/s13041-019-0510-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — MAP 1B phosphorylation at S25 and at S1201 are induced and maintained in the proximal segment of the transected sciatic nerve. a, b, c and d Immunofluorescent staining for pS25 (a), pS1201 (b), pan-MAP 1B (c), or SCG10 (d) with Tuj-1 Abs of intact nerve (left), transected nerve on 1 day (middle), and 3 days (right) after injury. On day 1 and day 3, the proximal and the distal segments were located in the left and the right side from the injury point. Scale bar; 200 μm. e, f, g and h Quantitative assessment of the signal intensity of pS25 (e), pS1201 (f), pan-MAP 1B (g), and SCG10 (h). N = 3; * p < 0.05, ** p < 0.01, and *** p < 0.001, by comparison with that in the intact nerve; using one-way ANOVA with Dunnett’s multiple comparisons test