Fig. 2.

Effect of HDGF on VEGF expression in oral cancer cells. SCC4 cells were treated with the indicated concentration of recombinant HDGF protein for 24 h before harvest. a Relative gene expression levels of VEGF were analyzed by SYBR green-based RT-PCR. Data are expressed as the fold change with respect to the control group (means ± SD of triplicate experiments). b Cell lysates were analyzed using Western blotting, and the protein levels of VEGF/β-actin were measured and quantified. c The secreted VEGF protein levels in the supernatants were measured by Western blotting. Ponceau S staining was used as a loading control. d Levels of secreted VEGF protein (pg/ml) were detected by enzyme-linked immunosorbent assay (ELISA) in triplicate experiments. Data were mean of three experiments. *, P < 0.05; **, P < 0.01; ns, not statistically significant