Fig. 5.

Effects of chetomin and Bay 11–7082 on HDGF-induced VEGF upregulation in SCC4 oral cancer cells. Cells were treated with recombinant HDGF protein (100 ng/ml) in the presence of Bay 11–7082 (10 nM) or chetomin (10 nM) for 24 h. a Relative gene expression levels of VEGF were analyzed by SYBR Green-based RT-PCR. Data are expressed as the fold change with respect to the control group (means ± SD of triplicate experiments). b The protein levels of VEGF were analyzed by Western blotting and normalized to β-actin expression. (c) the levels of secreted VEGF protein (pg/ml) were detected by ELISA in triplicate experiments. d Scheme for HDGF-regulated VEGF transcription in oral cancer cells. Data were mean of three experiments. *, P < 0.05; **, P < 0.01; ns, not statistically significant