Figure 1.

Systematic Enrichment of Aptamers against MS

Negative selection was performed by incubating the random ssDNA library (RDL) with nitrocellulose membrane (NCM) (step a) and then partitioning of bound and unbound sequences (step b); the unbound DNAs were collected and then incubated with immobilized MS for positive selection (step c); after washing, the bound DNAs were eluted and amplified by PCR for the next round of SELEX (step d); after enrichment, the DNA population was cloned and sequenced to identify the sequences of individual monoclonal aptamers (step e).