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Cardiac tissues from left ventricles were collected and then subjected to quantitative reverse-transcription polymerase chain reaction analysis with specific primers for HO-1 (A), NQO1 (B), catalase (C), glutamate-cysteine ligase (GCLC) (D), SOD1 (E), SOD2 (F), Grx1 (G), and Gpx1 (H). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control (n = 5 to 6, ±S.E.M.).
