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. 2019 Dec;371(3):642–651. doi: 10.1124/jpet.119.261792

TABLE 2.

Primer pairs used for quantitative reverse-transcription polymerase chain reaction analysis (qRT-PCR)

Gene Sequence Gene Bank Number
Nrf2 Forward: 5′-GCC​AGC​TGA​ACT​CCT​TAG​AC-3′ NM_031789.2
Reverse: 5′-GAT​TCG​TGC​ACA​GCA​GCA -3′
HO-1 Forward: 5′-CGA​CAG​CAT​GTC​CCA​GGA​TT-3′ NM_012580.2
Reverse: 5-TCG​CTC​TAT​CTC​CTC​TTC​CAG​G-3′
NQO1 Forward: 5′-CAT​TCT​GAA​AGG​CTG​GTT​TGA-3′ NM_017000.3
Reverse: 5′-CTA​GCT​TTG​ATC​TGG​TTG​TCG-3′
SOD1 Forward: 5′-GCG​GTG​AAC​CAG​TTG​TGG​TG-3′ NM_017050.1
Reverse: 5′-GCT​GGA​CCG​CCA​TGT​TTC​TT-3′
SOD2 Forward: 5′-AGG​AGA​GTT​GCT​GGA​GGC​TA-3′ NM_017051.2
Reverse: 5′-AGC​GGA​ATA​AGG​CCT​GTT​GTT-3′
CAT Forward: 5′-CGA​CCG​AGG​GAT​TCC​AGA​TG-3′ NM_012520.2
Reverse: 5′-CCT​GCC​TCT​TCA​ACA​GGC​AA-3′
GCLC Forward: 5′-GTG​GAC​ACC​CGA​TGC​AGT​AT-3′ NM_012815.2
Reverse: 5′-TCA​TCC​ACC​TGG​CAA​CAG​TC-3′
Grx1 Forward: 5′-CTG​TCA​GCA​TGG​CTC​AGG​AGT-3′ NM_022278.1
Reverse: 5′-CCA​CAA​ATT​CCA​GGA​GAC​CAC-3′
GPX1 Forward: 5′-CAC​AGT​CCA​CCG​TGT​ATG​CC-3′ NM_030826.4
Reverse: 5′-AAG​TTG​GGC​TCG​AAC​CCA​CC-3′
TNF-α Forward: 5′-AAA​TGG​GCT​CCC​TCT​CAT​CAG​TTC-3′ X66539.1
Reverse: 5′-TCT​GCT​TGG​TGG​TTT​GCT​ACG​AC-3′