TABLE 2.
Primer pairs used for quantitative reverse-transcription polymerase chain reaction analysis (qRT-PCR)
Gene | Sequence | Gene Bank Number |
---|---|---|
Nrf2 | Forward: 5′-GCCAGCTGAACTCCTTAGAC-3′ | NM_031789.2 |
Reverse: 5′-GATTCGTGCACAGCAGCA -3′ | ||
HO-1 | Forward: 5′-CGACAGCATGTCCCAGGATT-3′ | NM_012580.2 |
Reverse: 5-TCGCTCTATCTCCTCTTCCAGG-3′ | ||
NQO1 | Forward: 5′-CATTCTGAAAGGCTGGTTTGA-3′ | NM_017000.3 |
Reverse: 5′-CTAGCTTTGATCTGGTTGTCG-3′ | ||
SOD1 | Forward: 5′-GCGGTGAACCAGTTGTGGTG-3′ | NM_017050.1 |
Reverse: 5′-GCTGGACCGCCATGTTTCTT-3′ | ||
SOD2 | Forward: 5′-AGGAGAGTTGCTGGAGGCTA-3′ | NM_017051.2 |
Reverse: 5′-AGCGGAATAAGGCCTGTTGTT-3′ | ||
CAT | Forward: 5′-CGACCGAGGGATTCCAGATG-3′ | NM_012520.2 |
Reverse: 5′-CCTGCCTCTTCAACAGGCAA-3′ | ||
GCLC | Forward: 5′-GTGGACACCCGATGCAGTAT-3′ | NM_012815.2 |
Reverse: 5′-TCATCCACCTGGCAACAGTC-3′ | ||
Grx1 | Forward: 5′-CTGTCAGCATGGCTCAGGAGT-3′ | NM_022278.1 |
Reverse: 5′-CCACAAATTCCAGGAGACCAC-3′ | ||
GPX1 | Forward: 5′-CACAGTCCACCGTGTATGCC-3′ | NM_030826.4 |
Reverse: 5′-AAGTTGGGCTCGAACCCACC-3′ | ||
TNF-α | Forward: 5′-AAATGGGCTCCCTCTCATCAGTTC-3′ | X66539.1 |
Reverse: 5′-TCTGCTTGGTGGTTTGCTACGAC-3′ |