Table 1.
Crossing experiments
| Female ID | Biotype | Origin | Male ID | Biotype | Origin | Progeny | Family ID | ||
|---|---|---|---|---|---|---|---|---|---|
| Sexual | Clonal | Polyploid | |||||||
| EENF1 | EEN | Okna R., Slovakia | EEM1 | EE | Okna R., Slovakia | 0 | 6 | 1 | No. 401 |
| EENF24 | EEN | Okna R., Slovakia | EEM8 | EE | Okna R., Slovakia | 0 | 16 | 0 | No. 424 |
| EENF25 | EEN | Okna R., Slovakia | EEM8 | EE | Okna R., Slovakia | 0 | 9 | 0 | No. 425 |
| EENF10 | EEN | Ipeľ R., Slovakia | EEM9 | EE | Nová Říše, Czech R. | 0 | 9 | 0 | No. 413 |
| 097CENNF1 | EN | Jantra R., Bulgaria | 09EXPM7C | PP | Veleka R., Bulgaria | 0 | 6 | 4 | No. 8 |
| 10EXF1F9C08 | TP | Laboratory hybrid | 10EXF1M9C08 | TP | Laboratory hybrid | 11 | 6 | 9 | No. 1 |
| F1TFPM062 | TP | Laboratory hybrid | F1TFPM065 | TP | Laboratory hybrid | 6 | 0 | 0 | No. 17; clutch A |
| F1TFPM062 | TP | Laboratory hybrid | F1TFPM066 | TP | Laboratory hybrid | 1 | 0 | 0 | No. 17; clutch B |
Biotype—E, haploid Cobitis elongatoides genome; N, haploid C. tanaitica genome; P, haploid C. pontica genome; T, haploid C. taenia genome. For each family, we indicate different types of progeny: “sexual” denotes a number of progeny obtained from segregating gametes; “clonal,” a number of progeny obtained from clonal gametes; “polyploid,” a number of progeny obtained from fertilized clonal gametes. Note that several clutches from different F1 individuals occurred in the experimental family No. 17. GPS coordinates: Okna R. 48.718100, 22.120511; Ipeľ R. 48.072868, 19.088204; Nová Říše 49.152760, 15.547374; Jantra R. 43.469008, 25.725494; Veleka R. 42.026909, 27.623940.