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. 2019 Nov 5;10:2552. doi: 10.3389/fimmu.2019.02552

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Copyright © 2019 Herzog, Dach, de Buhr, Niemann, Schlagowski, Chaves-Moreno, Neumann, Goretzko, Schwierzeck, Mellmann, Dübbers, Küster, Schültingkemper, Rescher, Pieper, von Köckritz-Blickwede and Kahl.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CF sputum samples visualized by immunofluorescence staining and confocal microscopy. Staining of two sputa (A–D and E–H) of two different CF patients with chronic S. aureus infection. Nuclei and DNA structures (blue, A,E) and DNA-histone-1-complexes (red, B,F) are visible, whereas antibody-mediated staining specifically identifies S. aureus (green, C,G). Microscopy reveals NET-structures with S. aureus attached to it, but also with S. aureus without NET-binding (D,H). Subsequent changes of color balance for color intensification were performed with ImageJ.