Table 2.
Bacterial isolates from FSC135, FSC500 and FSC1000 and HC degradation capability. Origin culture denotes the treatment (NAP, PHE, PYR, PAH mix, CRUDE) each strain was isolated from. Testing for hydrocarbon degradation was performed using model oil as substrate, which contains a mixture of aliphatic and aromatic hydrocarbons
| Isolate id. (accession number) | Origin culture | Query length | Closest relative in SILVA database (accession number) | Origin of closest neighbour | Similarity (%) | Hydrocarbon degradation |
|---|---|---|---|---|---|---|
| 135‐A (MF977460) | NAP | 1358 | Belliela aquatica TS‐T86 (KC762321) | Culture of the alga Phaeodactylum tricornutum, China | 92% | + |
| 135‐B (MF977461) | CRUDE | 1384 | Aequorivita viscosa 81‐b (HM485318) | Intertidal zone, East China Sea | 97% | − |
| 135‐C (MF977462) | PYR | 1387 | Uncultured Halomonas sp. clone C146300069 (JX530673) | Southern ocean iron fertilization experiment (LOHAFEX) | 99% | + |
| 135‐D (MF977463) | PYR | 1394 | Halomonas sp. B01 (KJ778559) | Salt pond sediment of a seawater baysalt field | 99% | + |
| 135‐E (MF977464) | PYR | 943 | Uncultured Halomonas sp. clone C146300069 (JX530673) | 99% | + | |
| 135‐F (MF977465) | PYR | 948 | Uncultured Halomonas sp. clone C146300069 (JX530673) | 99% | + | |
| 135‐G (MF977466) | CRUDE | 1400 | Pseudomonas pelagia CL‐AP6 (AROI01000066) | Culture of Pyramimonas gelidicola from the Antarctic | 98% | ++ |
| 135‐H (MF977467) | NAP | 1341 | Ahrensia kielensis DSM 5890 (ARFW01000003) | 98% | ++ | |
| 135‐I (MF977468) | NAP | 1347 | Ahrensia kielensis NBRC 15762 (AB680960) | 98% | − | |
| 135‐J (MF977469) | NAP | 1299 | Sulfitobacter undariae strain W‐BA2 (KM275624) | Brown algae reservoir, South Korea | 99% | + |
| 135‐K (MF977470) | NAP | 1385 | Brumimicrobium sp. P99 (EU195945) | Hydrocarbon polluted sediments, Spain | 96% | − |
| 135‐L (MF977471) | CRUDE | 1376 | Kineosporiaceae bacterium G3A1 (KF994923) | 94% | + | |
| 135‐M (MF977472) | CRUDE | 1346 | Rhodococcus fascians strain Gert_13:10 (KR088385) | Groundwater | 100 | ++ |
| 500‐A (MF977473) | PHE | 1366 | Chryseobacterium sp. LW‐NC3 (KJ958494) | Wastewater | 98% | ++ |
| 500‐B (MF977474) | PHE | 1395 | Pseudomonas sp. gap‐f‐76 (DQ530477) | Crude oil‐contaminated Antarctic sea‐ice | 100% | + |
| 500‐C (MF977475) | PHE | 1392 | Pseudoalteromonas haloplanktis TB25 ctg310 (AUTI01000310) | Antarctic sponge | 100% | + |
| 500‐D (MF977476) | CRUDE | 1391 | Pseudoalteromonas haloplanktis TAE80 (AUTM01000360) | Antarctic water column | 100% | + |
| 500‐E (MF977477) | CRUDE | 1391 | Pseudoalteromonas haloplanktis TAE80 (AUTM01000360) | Antarctic water column | 100% | − |
| 500‐F (MF977478) | PYR | 1393 | Pseudoalteromonas elyakovii (AF082562) | 100% | + | |
| 500‐G (MF977479) | PHE | 1395 | Pseudoalteromonas haloplanktis TAE79 (AUTL01000217) | Antarctic water column | 100% | + |
| 500‐H (MF977480) | PAH | 1397 | Shewanella sp. 135Z‐7 (JX310137) | Southern Ocean | 100% | − |
| 500‐I (MF977481) | PYR | 1397 | Shewanella vesiculosa (AM980877) | Antarctic sediment | 100% | − |
| 500‐J (MF977482) | PHE | 1384 | Glaciecola polaris LMG 21857 (AJ29382) | Polar seas | 100% | − |
| 500‐K (MF977483) | CRUDE | 1321 | Sulfitobacter sp. PIC‐72 (AJ534241) | North Sea | 100% | + |
| 500‐L (MF977484) | CRUDE | 1326 | Sulfitobacter sp. BSw21498 (GQ358930) | Subarctic glacial fjord, Kongsfjorden | 100% | + |
| 500‐M (MF977485) | PYR | 1365 | Rhodococcus sp. R7704 (AJ295711) | Polar seas | 100% | ++ |
| 1000‐A (MF977486) | PAH mix | 1377 | Shewanella sp. JL‐56 (AY745827) | North pacific | 100% | − |
| 1000‐B (MF977487) | PAH mix | 1398 | Shewanella sp. R7216 (AJ295714) | Polar seas | 100% | − |
| 1000‐C (MF977488) | CRUDE | 1405 | Shewanella sp. 135Z‐7 (JX310137) | Southern Ocean | 100% | |
| 1000‐D (MF977489) | PAH mix | 1393 | Pseudoalteromonas haloplanktis TB13 (AUTJ01000235) | Antarctic sponge | 100% | − |
| 1000‐E (MF977490) | PAH mix | 1363 | Pseudoalteromonas sp. An83 (LN881340) | Surface microbiota of the brown alga Ascophyllum nodosum | 100% | + |
| 1000‐F (MF977491 | PAH mix | 1361 | Pseudoalteromonas sp. S8‐8 ctg46 (AUTR01000046) | Antarctic sediment | 100% | + |
| 1000‐G (MF977492) | CRUDE | 1366 | Pseudoalteromonas flavipulchra (JTDZ01000010) | Seawater, Nice, France | 100% | + |
| 1000‐H (MF977493) | CRUDE | 1376 | Pseudoalteromonas sp. BIc20004 (FJ748504) | Ny‐Alesund ice core | 100% | − |
| 1000‐I (MF977494) | CRUDE | 1376 | Pseudoalteromonas sp. An93 (LN881244) | Surface microbiota of the brown alga Ascophyllum nodosum | 100% | − |
| 1000‐K (MF977495) | CRUDE | 1384 | Pseudoalteromonas sp. B36 (AB607158) | North Sea seawater | 100% | + |
| 1000‐L (MF977496) | PYR | 1382 | Pseudoalteromonas sp. BSw20083 (EU365608) | Arctic seawater | 100% | − |
| 1000‐M (MF977497) | PHE | 1392 | Pseudoalteromonas elyakovii (AF082562) | 100% | − | |
| 1000‐N (MF977498) | CRUDE | 1381 | Colwellia sp. SS12.12 (KC160905) | Antarctic sediment | 99% | − |
| 1000‐O (MF977499) | PYR | 1391 | Glaciecola polaris LMG 21857 (AJ293820) | Polar Seas | 100% | − |
| 1000‐P (MF977500) | PYR | 1380 | Marinomonas sp. BSw10506 (EF437161) | Antarctic seawater | 98% | − |
| 1000‐Q (MF977501) | CRUDE | 1377 | Bizionia sp. KJF12‐2 (JQ800199) | Subarctic glacial fjord, Kongsfjorden | 98% | − |
| 1000‐R (MF977502) | CRUDE | 1344 | Bizionia sp. KJF12‐2 (JQ800199) | Subarctic glacial fjord, Kongsfjorden | 98% | − |
| 1000‐S (MF977503) | CRUDE | 1370 | Rhodococcus sp. NS8 (KM361868) | Nepheline sands from tailing dumps, Russia | 100% | ++ |