Figure 1.

GMP proliferation in integrin β2−/− mice. The absolute number of granulocyte (A), monocyte count (B), and lymphocyte (C) in the PB of integrin β2−/− and wild‐type mice. n = 9–12. (D): Bone marrow cells (BMC) were stained with anti‐CD11b and anti‐Gr‐1. The numbers of GR‐1⁺ granulocytes and CD11b⁺ monocytes were shown. n = 7 for each group. (E): BMC were stained with lineage cocktail, anti‐Sca‐1, anti‐cKit, anti‐CD16/32, and anti‐CD34. The numbers of HSPCs, CMP, GMP, and MEP in BMC were obtained. n = 11–13. (F): BMCs were permeabilized and stained with surface markers together with BrdU‐FITC. BrdU‐incorporating CMP and GMP were analyzed by FACS. The percentage of BrdU‐incorporating CMP or GMP within CMP or GMP population was shown. n = 6–7. (G): Representative BrdU+ cells when gated on lineage^‐/lowSca‐1⁻cKit⁺CD34⁺CD16/32⁺ cells, that is, GMP. # denoted BrdU+ cells. (H): Colony‐forming unit assay using 1 × 10⁴ BMC of β2+/+ and β2−/− mice. Ten days after plating, myeloid colonies were counted under light microscope. n = 8–9. (I): BMC were stained with anti‐lineage, anti‐cKit, anti‐CD135, anti‐CD115, anti‐Ly6C, and anti‐CD11b. GMP subpopulations were analyzed by FACS. cMoP: CD117⁺CD115⁺CD135⁺Ly6C⁺CD11b⁻lineage^−/low; MDP: CD117⁺CD115⁺CD135⁺Ly6C⁻CD11b⁻lineage^−/low; Ly6C^hi monocytes: CD117⁻CD115⁺CD135⁻Ly6C^hilineage−/low; and Ly6C^lo monocytes: CD117⁻CD115⁺CD135⁻Ly6C^lolineage^−/low. n = 8 for each group. Abbreviations: BrdU, 5‐bromo‐2‐deoxyuridine; cMoP, common monocyte progenitors; CMP, common myeloid progenitors; FACS, Fluorescence Activated Cell Sorting; FITC, Fluorescein isothiocyanate; GMP, granulocyte/macrophage progenitor; HSPCs, hematopoietic stem/progenitor cells; MDP, monocyte–macrophage DC progenitors; MEP, megakaryocyte/erythrocyte progenitor; PB, peripheral blood.