Figure 3.

Microarray analysis. (A): Equal amount of integrin β2+/+ bone marrow cells (BMC) or integrin β2−/− BMC was mixed with 1 × 10⁶ CD45.1 BMC and then injected into lethally irradiated CD45.1 recipient. Sixteen weeks after transplantation, blood cells were stained with anti‐CD45.1, anti‐CD45.2, and anti‐CD11b for myeloid lineage production. Likewise, blood cells were stained with anti‐CD45.1, anti‐CD45.2 and anti‐CD4, anti‐CD8 and anti‐B220 for chimerism analysis of lymphoid lineage production by FACS. n = 7–9. (B): β2+/+ and β2−/− CMP were sorted out by FACS and competitive transplantation was performed by injecting 1,000 CMP and 1 × 10⁵ CD45.1 BMC to irradiated CD45.2 recipient. Twelve days after injection, recipient's BMC were stained with anti‐CD45.1, anti‐CD45.2, and GMP markers for chimerism analysis. n = 5–6. (C): CMP were sorted out by FACS and proceeded for microarray analysis. Pathways enriched in β2−/− CMP as compared with β2+/+ CMP were listed by KEGG pathway analysis. n = 3 for each. qRT‐PCR analysis of FcεRIα in CMP (D) and GMP (E). Gene expression was normalized to β‐actin. n = 3–4. Abbreviations: CMP, common myeloid progenitors; FACS, fluorescence activated cell sorting; GMP, granulocyte/macrophage progenitor; MEP, megakaryocyte/erythrocyte progenitor.