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. 2019 Feb 7;37(3):430–440. doi: 10.1002/stem.2961

Figure 4.

Figure 4

Characterization of FcεRIαhi GMP in myeloid production. (A): The absolute numbers of FcεRIαhi CMP and FcεRIαhi GMP in β2+/+ and β2−/− mice. n = 12–13. (B, C): The absolute numbers of FcεRIαlo CMP and FcεRIαlo GMP in β2+/+ and β2−/− mice. n = 12–13. (D–F): Equal amount of FcεRIαhi GMP or FcεRIαlo GMP were mixed with 1 × 105 CD45.1 bone marrow cells (BMC) and then transplanted to lethally irradiated CD45.2 recipients. Twelve days after transplantation, blood cells were stained with anti‐CD.1, anti‐CD45.2, anti‐CD11b, and anti‐Gr‐1 for chimerism analysis by FACS. n = 12–15. (G‐I): Equal amount of FcεRIαhi GMP from β2+/+ and β2−/− mice were mixed with 1 × 105 CD45.1 BMC and then transplanted to lethally irradiated CD45.2 recipients. Chimerism analysis was performed at 12 days after transplantation. n = 4–5. (J, K): Equal amount of FcεRIαlo GMP from β2+/+ and β2−/− mice were mixed with 1 × 105 CD45.1 BMC and then transplanted to lethally irradiated CD45.2 recipients for chimerism analysis. n = 5 for each group. Abbreviations: CMP, common myeloid progenitors; FACS, fluorescence activated cell sorting; GMP, granulocyte/macrophage progenitor.