Figure 2.

Inhibition of O‐glycosylation decreases TRAIL sensitivity. COLO 205 (A), HCT 116 (B) and fibroblasts (C) were pretreated with 2 mm bGalNAc for 24 h, after which cells were stimulated with 1–1000 ng·mL ⁻¹ rhTRAIL 4C7 or DHER for 16 h. Cell death levels were determined by MTS assay. The values shown are mean ± SD of three independent experiments. P value was analysed by one‐way ANOVA in Turkey's multiple comparison with graphpad prism version 5.00. **P < 0.005 and ***P < 0.0005.