Figure 10.

Effects of recombinant JAG1 protein on Notch ligand and receptor gene expression in granulosa cells. Primary cultured granulosa cells were plated onto fibronectin (control) or immobilized recombinant Jag1 as described in the “Methods” section. (A) Expression of the Notch ligands Jag1 and Jag2 and the Notch receptors Notch2 and Notch3 was analyzed. (B) Expression of the ligand Jag1 was analyzed with or without a 24-h treatment with the Notch inhibitor DAPT. n = 3 for each condition, with individual experiments shown as points on the scatter plots. The bars designate mean ± SEM. In (A), *P < 0.05. In (B), means not sharing the same letter are significantly different (at P < 0.05).