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. 2019 May 3;112(1):81–98. doi: 10.1111/mmi.14255

Figure 3.

Figure 3

Hbp expression and processing in response to compound hits. A. E. coli MC1061 degP::S210A cells were grown in 96‐well plates and induced for Hbp(‐derivative) expression in the presence or absence (last two lanes) of 200 µM of the indicated VUF compounds. Subsequently, cells were collected and separated from spent medium by centrifugation and analyzed by SDS‐PAGE and Western blotting. The periplasmic protein OppA, part of the oligopeptide permease Opp, was used as loading control. B. Structure of VUF16749. C. Structure of VUF15259.