Figure 4.

VUF15259 stress induction and inhibition of Hbp secretion. E. coli TOP10F’ bacteria were grown in a 96‐well plate and Hbp was expressed from the pEH3 plasmid. Cells were exposed for 3 h to an increasing concentration of VUF15259 as indicated in the Figure. A. Whole cell lysates were analyzed with SDS‐PAGE and Western blotting. The whole cell lysate of empty vector (pEH3) E. coli cells treated with VUF15259 were also analyzed. B. The spent medium was TCA precipitated and analyzed by SDS‐PAGE and Coomassie staining. C. Stress was monitored using PrpoE‐mNG and PgroES‐mNG on the pUA66 plasmid. Error bars represent the standard deviation of triplicate samples.