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. 2019 May 3;112(1):81–98. doi: 10.1111/mmi.14255

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© 2019 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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VUF15259 compromises outer membrane integrity and increases vesicle release. A. E. coli TOP10F’ cells were grown in a 96‐well plate and treated with compound VUF15259 and uptake of NPN was measured after 3 h. As a positive control, PMB was added and immediately afterwards uptake of NPN was determined. The fold difference in fluorescence is shown compared to control (DMSO treated) cells with the error bars representing the standard deviation of triplicate samples. B. E. coli TOP10F’ cells were grown to steady‐state and transferred to a 96‐well plate for the treatment with 100 µM VUF15259 or DMSO as control. After 3 h of growth, cells were collected, washed and analyzed by TEM using EPON sectioning and negative staining (uranyl acetate). Scale bars are indicated in the figure. The arrow indicates a potential membrane disruption.