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. 2019 Mar 12;43(5):516–527. doi: 10.1002/cbin.11117

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© 2019 The Authors. Cell Biology International Published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Formation of gamma‐H2AX foci in zebrafish embryos after irradiation at early developmental stages. (A) Fluorescein immunostaining images of anti‐γH2AX antibody (green, left panels), Hoechst 33342 (blue, middle panels) and merged images (right panels) in control embryos (upper panels) and 1 Gy‐irradiated embryos (lower panels) at 6 hpf. Irradiated samples showed γH2AX foci within nuclei, whereas controls have almost no γH2AX foci. (B) Embryos irradiated with 1 Gy at 2, 4, or 6 hpf were fixed at 15‐min intervals and stained with an anti‐γH2AX antibody. The numbers of foci per nuclei at each timepoint were plotted as: 2 hpf control (light blue) and 1 Gy irradiated (orange), 4 hpf control (yellow), and 1 Gy irradiated (blue) or 6 hpf control (dark blue) and 1 Gy irradiated (brown). Only the 6 hpf embryos irradiated at 1 Gy showed γH2AX foci formation. Foci numbers peaked at 30 min after irradiation.