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. 2019 Mar 12;43(5):516–527. doi: 10.1002/cbin.11117

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© 2019 The Authors. Cell Biology International Published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Effect of radiation exposure on M‐phase cells in zebrafish embryos at early developmental stages. (A) Immunostaining images of anti‐pHH3 antibody (green, left panels), Hoechst 33342 (blue, middle panels) and merged images (right panels) from embryos of 0 h after control (upper panels) or 1 Gy irradiation (lower panels) at 6 hpf. (B) Irradiated embryos were fixed at 1‐h intervals. The M‐phase marker, phospho‐histone H3 (phospho‐HH3) was detected by immunostaining; the ratio of positive cells/nuclei at each timepoint was calculated. In embryos irradiated at 4 hpf, the number of cells in M‐phase did not change compared to those in the control embryos (blue bars). However, cells irradiated at 6 hpf (orange bars) showed a reduction in M‐phase cells immediately after irradiation (P = 0.06). The columns and error bars represent the mean and SD, respectively.