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. 2019 Mar 12;43(5):516–527. doi: 10.1002/cbin.11117

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© 2019 The Authors. Cell Biology International Published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Levels of p21 mRNA expression in irradiated zebrafish embryos. RNA samples were extracted 1 or 2 h after irradiating the embryos at 2, 4, or 6 hpf. Levels of p21 mRNA were measured via qPCR and normalized to those in control embryos at each timepoint. At all timepoints, the levels of p21 mRNA were upregulated in irradiated embryos. At all timepoints, 1 and 2 h after 2 hpf (P = 0.008 and P = 0.001), 2 h after 4 hpf (P = 0.02), and 2 h after 6 hpf (P = 0.005) were significantly different compared to the control at each timepoint. Note that upregulation of p21 mRNA levels in embryos irradiated at 2 hpf were much higher than those irradiated at other time points. The columns and error bars represent the mean and SD, respectively.