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. 2019 Mar 12;43(5):516–527. doi: 10.1002/cbin.11117

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© 2019 The Authors. Cell Biology International Published by John Wiley & Sons Ltd on behalf of International Federation of Cell Biology

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Levels of P21 protein expression in irradiated zebrafish embryos. A) Newly synthesized antibody recognized a protein between 20 and 25 kD in size. The amount of this protein decreased in the P21 antisense morpholino oligo (MO)‐injected embryos compared to embryos injected with standard control MO. B) Protein samples were obtained 1 or 2 h after irradiating embryos at 2, 4, or 6 hpf. In embryos irradiated at 6 hpf, the levels of P21/WAF1 protein were upregulated at 1 h after irradiation, whereas in embryos irradiated at 4 hpf, P21/WAF1 protein levels were upregulated only after 2 h.