Table 1.
Primers used for amplification of the follicle‐stimulating hormone receptor of Dongxiang and Suken chickens
| Primer | Primer sequence | Tm/°C | Product size/bp | Application |
|---|---|---|---|---|
| P1 | F:GGTATGGCTTACGCTTGTCTGT | 62 | 790 | Amplification |
| R:GATTGTTTGCTTGTTTCTTTCG | ||||
| P2 | F:AAAGGTGAGAATGGTGGAAT | 59 | 553 | Amplification |
| R:CCAGAGCTAAATAACGCACC | ||||
| P3 | F:AAAGGTGGTAGGGAGGAAGA | 62 | 740 | Amplification |
| R:CCTGGCAGATGAATATCCTG | ||||
| P4 | F:CGGggtaccACTCCCGTTCTTATGACACCTAT | 61 | 1,461 | Plasmids construction |
| R:CCCaagcttTTGTCTCCTTCTCCTCCATC | ||||
| P5 | F:CGGggtaccTTCTTGAACCTGTACCTCTTG | 61 | 794 | Plasmids construction |
| R:CCCaagcttTTGTCTCCTTCTCCTCCATC | ||||
| P6 | F:CGGggtaccTGGATCTATGAAGGGGAGC | 61 | 526 | Plasmids construction |
| R:CCCaagcttTTGTCTCCTTCTCCTCCATC | ||||
| P7 | F:GGTATGGCTTACGCTTGTCTGT | 62 | 790 | Genotyping |
| R:GATTGTTTGCTTGTTTCTTTCG | ||||
| P8 | F:TGTCTCTTAGTCTTATCAAACAACA | 60 | 492 | Genotyping |
| R:CCTGGCAGATGAATATCCTG | ||||
| P9 | F:AAAGGTGGTAGGGAGGAAGA | 62 | 740 | Genotyping |
| R:CCTGGCAGATGAATATCCTG | ||||
| P10 | F:ACAATCAAAACCCCAGCAAC | 62 | 741 | Genotyping |
| R:AATGAACCGGAATGCTTTTG |
The digestion sites of the enzymes are underlined.