Figure 7.
Class II CdiA toxin delivery works cross‐species. A & B. Average competitive index of cells expressing CdiA F11 from a ColE1 plasmid after co‐culturing with Salmonella typhimurium E. coli CFT073, UPEC 536, Enterobacter cloacae, Klebsiella pneumoniae or Enterobacter aerogenes with (light green bars) or without (dark green bars) CdiI expressed from a CloDF plasmid. Co‐culturing was performed for 5 h in liquid LB media (A) or 24 h on solid M9Glu media (B) (n = 4 biological replicates). C. CdiAF11 expressing S. typhimurium LT2 competed against S. typhimurium LT2 with (light grey bars) or without (dark grey bars) CdiI immunity on liquid LB (green) or solid M9Glu (purple) media (n = 4 biological replicates). Individual data points of the biological replicates are shown as black and white circles. [Colour figure can be viewed at https://www.wileyonlinelibrary.com]