Figure 3.

BP, PP, PhP, and TBP reduce tyrosinase protein levels, while the other phenols do not (a) Immunoblot showing tyrosinase protein levels (upper panel) in Mel 88.23 cells exposed for 72 hr to the indicated phenols (IC25 concentration) compared to untreated Mel 88.23 cells. Human keratinocytes (HaCaT cells) serve as negative control for tyrosinase expression. The two black bars indicate different tyrosinase forms (a mature 80‐kDa and an immature 70‐kDa form; Halaban, Cheng, Svedine, Aron, & Hebert, 2001). Actin immunostaining was performed as a loading control (lower panel). (b) Similar analyses as (a) for the effect of HOP, PP, PhP and TBP