Figure 6.

Subcutaneous delivery of TGF-β-coupled Ag-NPs reduces the severity of relapse symptoms in EAE. SJL mice were injected s.c. with 1.25 mg of Ag-NPs (8 μg Ag/mg, 166 ng TGF-β/mg) 7 days before immunization with PLP/CFA to induce EAE (n = 5). Statistical differences between treatments for the indicated time periods were determined by the Kruskal–Wallis test (one-way ANOVA nonparametric test) with Dunn’s multiple comparisons test (p < 0.05).