Figure 6.

Proximal regulatory region is involved in Fgf23 expression induced by LPS. (A) FGF23-PKO (PKO), (B) FGF23-38KO (38KO), and (C) FGF23-IKO (IKO) mice and their wild-type (WT) littermates were treated with either vehicle (Veh) or 10 mg/kg bw of LPS for 6 h. Levels of serum iFGF23 and Fgf23 and Il6 expression in the indicated tissues are presented. Gene expression level represents relative quantitation (RQ) normalized to Gapdh (ΔΔCT). Each value is the average of six to eight mice per group, and error bars represent SEM. *P < 0.05 compared with vehicle controls; ^#P < 0.05 compared with WT littermates that received the same treatments. Fgf23 levels were undetectable by RT-PCR. UD, undetectable.