Table 1.
Oligonucleotides for Genotyping
| Mutant Strains | Primers for Genotyping | Alleles (Size) |
|---|---|---|
| FGF23-PKO | TTGCTCCTGTCAAGTATGGAACA | WT (435 bp) |
| GTGAGAAGGAGGGCGTTTTGG | ||
| TTGCTCCTGTCAAGTATGGAACA | KO (284 bp) | |
| CAGGAAAAACAATGCCGCCAC | ||
| FGF23-38KO | CGACTGGGTTATGAGTTTCTTGG | WT (350 bp) |
| CCTGCTTAGGCTGTAGTGATGAG | ||
| ACAATAAGACTCCTCCCTTGGATG | KO (317 bp) | |
| CATTTTAAGGGCGAGGAAAGTGC | ||
| FGF23-IKO | GGTTGAAGATGAATGTTAGTCTAGTC | WT (556 bp) |
| GGCGAACAGTGTAGAAATGCAG | ||
| GGTTGAAGATGAATGTTAGTCTAGTC | KO (271 bp) | |
| GGCGAACAGTGTAGAAATGCAG | ||
| FGF23-16KOa | ACGCCCTTTAGCCCAGTAAT | WT (537 bp) |
| ATGGTGGGAAGGTGTGGTAA | ||
| AACAGTGTGAAGGGGTCCTG | KO (600 bp) | |
| TACCCACGAGGAACATGTGA |
a
Previously generated (31).