Table 1.
Kinetic parameters of enzymatic activity of Synechococcus WH7803 and cyanophage S‐PM2 MazG protein.
| V max (nmol/μg/min) | K m (mM) | K cat (min−1) | ||||
|---|---|---|---|---|---|---|
| Synechococcus sp. WH7803 | Cyanophage S‐PM2 | Synechococcus sp. WH7803 | Cyanophage S‐PM2 | Synechococcus sp. WH7803 | Cyanophage S‐PM2 | |
| dATP | 1.8 (±0.28) | 1.62 (±0.19) | 0.3 (±0.09) | 1.2 (±0.21) | 126.12 (±19.35) | 62.97 (±7.44) |
| dCTP | 3.81 (±0.36) | 8.86 (±0.2) | 0.14 (±0.03) | 1.16 (±0.04) | 267.68 (±25.02) | 344.68 (±7.72) |
| dTTP | 6.57 (±0.19) | 5.68 (±0.2) | ND | 1.23 (±0.06) | 461.04 (±13.43) | 221.00 (±7.78) |
| dGTP | 0.64 (±0.25) | 10.29 (±0.25) | 0.85 (±0.07) | 0.14 (±0.01) | 45.16 (±17.6) | 400.35 (±9.91) |
| ATP | 2.55 (±0.35) | 2.28 (±0.24) | 0.63 (±0.23) | 1.43 (±0.36) | 179.27 (±24.4) | 88.7 (±9.41) |
| CTP | 1.96 (±0.14) | 2.51 (±0.17) | 1.2 (±0.21) | 0.85 (±0.11) | 137.81 (±9.81) | 97.48 (±6.68) |
| GTP | 0.7 (±0.13) | 0.3 (±0.02) | 0.26 (±0.02) | ND | 49.46 (±9.19) | 11.67 (±0.6) |
| UTP | 3.02 (±0.2) | 3.31 (±0.18) | 1.33 (±0.3) | 0.6 (±0.37) | 221.07 (±6) | 128.75 (±7.12) |
| dUTP | ‐ | 4.22 (±0.34) | ‐ | 3.24 (±1.55) | ‐ | 296.42 (±23.72) |
| 2‐hydroxy d‐ATP | ‐ | 1.65 (±0.06) | ‐ | 4.86 (±1.13) | ‐ | 115.65 (±3.95) |
| 8‐oxo‐dGTP | ‐ | ND | ‐ | ND | ‐ | ND |
The values in brackets represent SE based on three replicates. ND – not detected; − not measured.