Skip to main content
. 2019 Mar 1;98(3):479–491. doi: 10.1111/tpj.14231

Figure 7.

Figure 7

Expression analysis of DNA damage response (DDR) genes after short‐term and long‐term Al treatments.

(a) RNA was prepared from 10‐day‐old wild‐type (WT), atm, atr and sog1 seedlings, either untreated or treated with 1.5 mm Al‐containing hydroponics (pH 4.2) for 3 h. Relative expression levels of the indicated genes are shown as mean values from three biological repeats and with the untreated WT value set as 1. Error bars indicate SD. Significant differences from untreated value were determined by one‐way anova analysis, P < 0.05. (b) RNA was prepared from seeds germinated and grown on soaked gel medium containing 0 and 1.5 mm Al (pH 4.2) for 3 days. Relative expression levels of the indicated genes are shown as mean values from three biological repeats and with the untreated WT value set as 1. Error bars indicate SD. Significant differences from untreated plants were determined by one‐way anova analysis, P < 0.05. (c) RNA was prepared from seeds germinated and grown on soaked gel medium containing 0 and 1.5 mm Al (pH 4.2) for 10 days. Relative expression levels of the indicated genes are shown as mean values from three biological repeats and with the untreated WT value set as 1. Error bars indicate SD. Significant differences from untreated value were determined by one‐way anova analysis, P < 0.05.