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. 2019 Mar 7;34(6):1129–1142. doi: 10.1002/jbmr.3680

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© 2019 The Authors. Journal of Bone and Mineral Research Published by Wiley Periodicals, Inc.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Wnt1 increased the nuclear accumulation of β‐catenin in neighboring cells. (A) Immunofluorescence staining for Wnt1 (red) and β‐catenin (green) in control C3H10T1/2‐EV cells, mitomycin C‐treated Wnt1 overexpressing C3H10T1/2 cells or mixed culture of mitomycin C‐treated Wnt1 overexpressing C3H10T1/2 cells with control cells in 1:10 ratio. (B) Quantification of the β‐catenin fluorescence intensity in the nuclear and the cytoplasmic compartments, presented as a ratio of nuclear intensity to cytoplasmic intensity. Nonparametric values were analyzed with the Steel‐Dwass method. Data are presented as mean ± SD (n = 10). *p < 0.05, **p < 0.01, ***p < 0.001.